Carolyn Suzuki, Ph.D.
Associate Professor

Dr.Carolyn Suzuki

Dr. Suzuki received her Ph.D. (1992) from the Johns Hopkins University conducting her thesis research with Richard D. Klausner, M.D. at the National Institutes of Health, USA. She was a post-doctoral fellow with Gottfried Schatz, Ph.D. at the Biozentrum in Basel, Switzerland. Dr. Suzuki received a Damon Runyon Cancer Research Foundation fellowship and a Basil O'Connor Scholars Award. Her research focuses on the function of ATP-dependent proteases and chaperones in mitochondrial homeostasis, cell signaling and apoptosis.

Publications:

Bernstein, S.H., Venkatesh, S. Li, M., Lee, J., Lu B., Hilchey, S.P., Morse, K.M., Metcalf, H.M., Andreeff, M., Brookes, P.S. and Suzuki, C.K., The mitochondrial ATP-dependent Lon protease: a novel drug target in lymphoma death mediated by the synthetic triterpenoids CDDO and its derivatives. Blood. 119:3321 (2012).

Sundararajan, V., Lee, J., Singh, K., Lee I., and Suzuki, C.K. Multitasking in the mitochondrion by the ATP-dependent Lon protease. Biochim. Biophys. Acta. Invited review on AAA+ proteins. 1823:56 (2012).

Fishovitz, J., Frase, H., Hudak, J., Li, M., Craig, S., Ko, K., Berdis, A., Suzuki, C.K., Lee, I. Active-site directed chemical tools for profiling mitochondrial Lon protease. ACS Chemical Biology 6:781 (2011).

Lee, J., Sundararajan, V., Lee I., and Suzuki, C.K., The mitochondrial ATP-dependent Lon protease. Handbook of Proteolytic Enzymes, Oxford, UK, 2011.

Antes, A., Inger, T., Chung, S., Lim, R., Lu, B., Parrott, A.M., Hill, H., Suzuki, C.K., and Lee, C.G., Differential regulation of full-length genome and a single-stranded 7S DNA along the cell cycle in human mitochondria. Nucleic Acids Res., 38(19):6466-76 (2010).

C. K. Suzuki. Protein Quality Control in Mitochondria, in Handbook of Cell Signaling, Ed. S. Subramani and M. Farquar, Elsevier (Amsterdam) (2008).

S.H. Chen, C.K. Suzuki and S.H. Wu. Thermodynamic Characterization of Specific Interactions between Human Lon Protease and G-quartet DNA. 36:1273 (2008)

Z. Granot, O. Kobiler, N. Melamed-Book, S. Eimerl, B. Lu, S. Brown, C.K. Suzuki, A.B. Oppenheim, A.B. and J. Orly. Turnover of mitochondrial Steroidogenic Acute Regulatory (StAR) Protein by Lon Protease: the Unexpected Effect of Proteasome Inhibitors. Molec. Endocrinol., 21:2164-77. (2007).

B. Lu, S. Yadav, P.G. Shah, T. Liu, B. Tian, S. Pukszta, N. Villaluna, E. Kutejova, C.C. Newlon, J.H. Santos, and C.K. Suzuki. Roles for the human ATP-dependent Lon protease in mitochondrial DNA maintenance. J. Biol. Chem. 282:17363 (2007).

I. Lee, A.J. Berdis and C.K. Suzuki. Recent Developments in the Mechanistic Enzymology of the ATP-Dependent Lon Protease from Escherichia coli: Highlights from Kinetic Studies. Molec. BioSystems. 2:477 (2006).

B. Lu, N. Garrido, J.N. Spelbrink and C.K. Suzuki. Tid1 isoforms are mitochondrial DnaJ-like proteins with unique carboxy-termini that determine cytosolic fate. J. Biol. Chem. 281:13150 (2006).

G. Ondrovicova, T. Liu, K. Singh, B. Tian, H. Li, O. Gakh, D. Perecko, J. Janata, Z. Granot, J. Orly, E. Kutejova and C.K. Suzuki. Cleavage site selection within a folded substrate by the mitochondrial ATP-dependent Lon protease. J. Biol. Chem. 280:25103 (2005).

H. Otto, C. Conz, P. Maier, T. Wölfle, C.K. Suzuki, P. Jenö, P. Rücknagel, J. Stahl, and S. Rospert. The chaperones MPP11 and Hsp70L1 form the mammalian ribosome-associated complex (mRAC). Proc. Natl. Acad. Sci. U S A. 102:10064 (2005).

T Liu, B. Lu, I. Lee, G. Ondrovicova, E. Kutejova and C.K. Suzuki. DNA and RNA binding by the mitochondrial Lon protease is regulated by nucleotide and protein substrate. J. Biol. Chem. 279:13902-13910 (2004).

B. Lu, T. Liu, J.A. Crosby, J. Thomas-Wohlever, I. Lee and C.K.Suzuki. The ATP-dependent Lon protease of Mus musculus is a DNA-binding protein that is functionally conserved between yeast and mammals. Gene, 306:45-55 (2003).

H. Stahlberg, E. Kutejova, K. Suda, B. Wolpensinger, A. Lustig, G. Schatz, A. Engel, C.K. Suzuki. Mitochondrial Lon of Saccharomyces cerevisiae is a ring-shaped protease with seven flexible subunits. Proc. Natl. Acad. Sci., USA 96: 6787 (1999).

J. M. van Dijl, E. Kutejova, K. Suda, D. Perecko, G. Schatz and C.K. Suzuki, The ATPase and protease domains of yeast mitochondrial Lon: Roles in proteolysis and respirationdependent growth. Proc. Natl. Acad. Sci. USA, 18:10584 (1998).

C. K. Suzuki, M. Rep, J. M. van Dijl, K. Suda, L. A. Grivell and G. Schatz. ATP-dependent Proteases that also Chaperone Protein Biogenesis. Trends Biochem. Sci.. 22:118 (1997).

M. Rep, J. M. van Dijl, K. Suda, G. Schatz, L. A. Grivell and C. K. Suzuki. Promotion of Mitochondrial Membrane Complex Assembly by a Proteolytically Inactive Yeast Lon. Science 274:103 (1996).

C. K. Suzuki, E. Kutejova and K. Suda, Analysis and Purification of ATP-dependent Mitochondrial Lon Protease of Saccharomyces cerevisieae. Meth. Enzymol. 260: 486 (1995).

C. K. Suzuki, K. Suda, N. Wang and G. Schatz. Requirement of the Yeast Gene LON in Intramitochondrial Proteolysis and Maintenance of Respiration. Science 264: 273 (1994).